Fig 1
Starting Reagents and Materials
- 10 g piperine on filters
- 250-300 ml EtOH 88%
- 50 ml EtOH 40%
- Beakers
- Filter paper
- Funnel
- Heater
Fig 2
Ethanol is added to piperine at heating to 40-50℃ Fig 3
Fig 3
Ethanol should turn yellow to brown in color Fig 4
Fig 4
Ethanol is stopped being added when the solution no colored. The warm solution of piperine in ethanol is filtered through a paper filter Fig 5
Fig 5
After filtration, the total volume of the piperine solution in ethanol is 250-300 ml Fig 6
Fig 6
The filtered solution is evaporated to a volume of 50-100 ml at T = 50-60℃ Fig 7
Fig 7
When the first crystals appear, stop evaporating. Fig 8
Fig 8
The evaporated solution is left to crystallize at room temperature overnight Fig 9
Fig 9
The resulting crystals are transferred to a porous glass filter (porosity 100; filters with smaller pores become clogged) and washed with a 50 ml portion of 40% ethanol Fig 10
Fig 10
The crystals are filtered out Fig 11
Fig 11
The filtered and washed crystals are dried on the filter Fig 12
Fig 12
Yield of crystalline piperine is 7.25 g or 72.5% Fig 13
Fig 13
